猪A型塞内卡病毒分离鉴定及其VP1基因变异分析

doi:10.11843/j.issn.0366-6964.2019.09.025

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (9): 1945-1950.doi: 10.11843/j.issn.0366-6964.2019.09.025

猪A型塞内卡病毒分离鉴定及其VP1基因变异分析

李秀博^1,2,3, 刘存^2,3, 崔玉东¹, 梁琳^2,3, 张建伟⁴, 崔尚金^1,2,3*

1. 黑龙江八一农垦大学动物科技学院, 大庆 163319;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
3. 农业部兽用药物与兽医生物技术北京科学观测实验站, 北京 100193;
4. 北京市畜牧总站, 北京 100107

收稿日期:2019-03-11 出版日期:2019-09-23 发布日期:2019-09-23
通讯作者: 崔尚金,主要从事动物传染病病原学与流行病学研究,E-mail:cuishangjin@caas.cn
作者简介:李秀博(1993-),女,辽宁铁岭人,硕士,主要从事动物病原与分子流行病学研究
基金资助:
中国农业科学院创新工程项目（ASTIP-IAS15）；国家重点研发项目（2016YFD0501003；2017YFD0502300）

Isolation and Identification of Porcine Senecavirus A and Mutation Analysis on Its VP1 Gene

LI Xiubo^1,2,3, LIU Cun^2,3, CUI Yudong¹, LIANG Lin^2,3, ZHANG Jianwei⁴, CUI Shangjin^1,2,3*

1. College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology of Ministry of Agriculture, Beijing 100193, China;
4. Beijing Livestock and Veterinary Station, Beijing 100107, China

Received:2019-03-11 Online:2019-09-23 Published:2019-09-23

摘要/Abstract

摘要： 2017年7月，南方某猪场发生不明病原引起的猪水疱性疾病。为了确诊本次水疱性疾病的病因及了解病原的遗传演化关系，笔者通过RT-PCR方法对引起该场水疱性疾病的病原进行检测，利用BHK21细胞分离病毒，RT-PCR、电镜观察等方法对分离病毒进行鉴定，同时对其主要的抗原基因VP1进行比对分析。RT-PCR检测结果表明导致该猪场发生水疱性疾病的病原为A型塞内卡病毒（Senecavirus A，SVA），将利用BHK21细胞分离并经鉴定的病毒命名为CH/FuJ1/2017株。同源性分析结果显示，CH/FuJ1/2017株与报道的SVV-001株的相似性最低，与分离自美国的USA-GBI29-2015株的相似性最高。基于SVA VP1基因氨基酸序列的分析结果表明，SVA分离株形成一个独立的小分支，与SVV-001株相比，SVA分离株在VP1蛋白的59、62、63、93、97、172位氨基酸存在突变，且位于VP1蛋白的B-C环上和CD-Ⅱ环上，这可能与SVA的细胞嗜性改变有关。综上表明我国SVA分离株具有遗传多样性，部分国内分离株与国外SVA分离株间具有密切的相关性。

Abstract: In July, 2017, an outbreak of swine vesicular disease caused by unknown pathogen in a pig farm in southern China. In order to confirm the pathogen of the vesicular disease and understand its genetic evolution, the pathogens associated with vesicular disease detected by RT-PCR and virus isolation, electron microscopy test and the phylogenetic analysis of VP1 gene were performed. RT-PCR results indicated that Senecavirus A (SVA), responsible for the vesicular disease occurred in the farm in southern China, was detected. SVA were successfully isolated in BHK21 cells and named CH/FuJ1/2017 strain. The SVA isolates had the lowest homology with the prototype SVV-001 strain and the highest homology with USA-GBI29-2015 strain from the United States. The analysis based on the amino acid sequences of SVA VP1 gene showed that isolates of SVA were located in a relatively independent branch and compared with SVV-001 strain, there were six amino acids mutations in VP1 in 59, 62, 63, 93, 97 and 172, respectively. These amino acids mutations mainly located on BC loop and CD loop Ⅱ on VP1. In conclusion, this study showed that SVA isolates in China were genetic diversity, and there was close correlation among some SVA isolates from China and foreign SVA isolates.

中图分类号:

S852.659.6

李秀博, 刘存, 崔玉东, 梁琳, 张建伟, 崔尚金. 猪A型塞内卡病毒分离鉴定及其VP1基因变异分析[J]. 畜牧兽医学报, 2019, 50(9): 1945-1950.

LI Xiubo, LIU Cun, CUI Yudong, LIANG Lin, ZHANG Jianwei, CUI Shangjin. Isolation and Identification of Porcine Senecavirus A and Mutation Analysis on Its VP1 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1945-1950.

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猪A型塞内卡病毒分离鉴定及其VP1基因变异分析

Isolation and Identification of Porcine Senecavirus A and Mutation Analysis on Its VP1 Gene

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